Macrophages, T-Cells and Endometriosis

by endosolu

Firstly to understand this abstract you need to understand macrophages and t-cells…

From Wikepedia… Macrophages (Greek: big eaters, from Greek μακρος (makros) = large, φαγειν (phagein) = to eat[1]) are a type of white blood cell that engulfs and digests cellular debris, foreign substances, microbes, cancer cells, and anything else that does not have the types of proteins specific of healthy body cells on its surface[2] in a process called phagocytosis.

From Wikepedia… A T cell, or T lymphocyte, is a type of lymphocyte (a subtype of white blood cell) that plays a central role in cell-mediated immunity

My read of this tells me that ‘your body’ realises it has endometriosis thats not meant to be where it is and increases the macrophages and t-cells to combate it. Makes perfect sense as this is what the body is designed to do…  the real issue is do you have the right proteins in your diet to make these macrophages and t-cells (probably not), is your liver clean enough to synthesise some proteins you might need to manufacture these (probably not)

The endometriosis protocol I suggest makes sure all these very basic needs are being met… read more here

Rediscovering peritoneal macrophages in a murine endometriosis model


STUDY QUESTION What are the features of peritoneal macrophage subgroups and T helper cells in the development of murine endometriosis?

SUMMARY ANSWER During the development of endometriosis in a murine model, large peritoneal macrophages (LPMs) and small peritoneal macrophages (SPMs) are polarized into M1 and M2 cells, respectively, and the proportions of T helper (Th) 1, Th17 and T regulatory (Treg) cells are increased.

WHAT IS KNOWN ALREADY Numerous studies investigating the etiology and pathogenesis of endometriosis have focused on the polarization states of peritoneal macrophages in endometriosis models and patients, but the results are inconclusive. Further studies indicate that peritoneal macrophages are composed of two distinct subsets: LPMs and SPMs, although their roles in endometriosis are unknown.

STUDY DESIGN, SIZE, DURATION This study involves a prospective and randomized experiment. Fifty C57BL/6 female mice were randomly allocated to five control and five experimental groups (n = 5/group) according to the presence or absence of transplantation. The transplant periods are 0.25, 3, 14, 28 and 42 days.

PARTICIPANTS/MATERIALS, SETTING, METHODS C57BL/6 mice were utilized to establish an endometriosis model by i.p. injection of allogeneic endometrial segments. Dynamic changes of peritoneal macrophage subsets and polarization profiles were evaluated by flow cytometry (FCM). Macrophage morphology and density were assessed by cell counting under a microscope. Dynamic changes of Th1, Th2, Th17 and Treg cells were estimated by FCM.

MAIN RESULTS AND THE ROLE OF CHANCE Peritoneal macrophages are composed of two distinct subsets: LPMs and SPMs. The proportion of SPMs increased immediately after peritoneal injection of endometrial tissues, whereas LPMs showed an opposite trend. Peritoneal macrophages differentiated into both M1 and M2 macrophages. The bidirectional polarization of macrophages was caused by the inverse trends of polarization of LPMs and SPMs. Consistently, the proportions of Th1, Th17 and Treg cells were all increased in mice with endometriosis.

LIMITATIONS, REASONS FOR CAUTION In this study, detection was only performed in a murine endometriosis model. Clinical data and more intervention experiments are required in understanding the roles of LPMs and SPMs in endometriosis.

WIDER IMPLICATIONS OF THE FINDINGS The dramatic changes of LPMs and SPMs in proportion and polarization profiles clarified the varying differentiation states of peritoneal macrophages. In addition, LPMs and SPMs may play different roles in the pathogenesis of endometriosis in different stages of endometriosis. Therefore, the new classification should be included in future relevant basic and clinical studies on endometriosis.

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